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Readouts most often include glycerol release and free fatty acid quantification in the supernatant, alongside cellular ATP or viability measures to confirm that observed lipolysis reflects genuine metabolic signaling rather than cytotoxic membrane disruption

Frank, S.J., et al

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(2006) A single-center, double-blinded, randomized controlled study to evaluate the relative efficacy of sublingual and oral vitamin B-complex administration in reducing total serum homocysteine levels